Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Lavras, MG, Brazil.
Plant Foods Hum Nutr. 2013 Sep;68(3):268-73. doi: 10.1007/s11130-013-0365-x.
The effect of coffee on cardiovascular diseases is still controversial. It is known that the process of decaffeination may influence the chemical constitution and, therefore, the biological effects of coffee. This study thus evaluated the effects of decaffeination on the levels of total phenols and chlorogenic acids in Coffea arabica L. samples, as well as the effects of ingesting both integral and decaffeinated coffee on the lipid profile and hemostatic and hematological parameters in normal and hyperlipidemic rats. Samples of integral and decaffeinated lyophilized coffee (Coffea arabica L., planted in Brazil) were used for chemical analysis (total phenols, chlorogenic acid and caffeine contents). For the bioassays, coffee beverages were prepared with non-lyophilized samples (10% w/v) and were filtered and administered to animals by gavage (7.2 mL/kg/day) over 30 days. On the 31st day after beginning the treatment with coffee beverages, hyperlipidemia was induced to the animals by administering Triton WR-1339 (300 mg/kg body weight). On day 32, blood was taken to determine the lipid profile, platelet aggregation, prothrombin time, partially activated thromboplastin time and hemogram. The contents of both phenolic compounds and chlorogenic acid in the integral coffee beverage were significantly lower than those in the decaffeinated coffee beverage. The animals treated with Triton WR-1339 presented a mixed hyperlipidemia. Although the decaffeination process caused a relative increase in total phenols and chlorogenic acids, the coffee drinks were unable to change the lipid profile or the hemostatic and hematological parameters in the studied animals.
咖啡对心血管疾病的影响仍存在争议。已知脱咖啡因过程可能会影响咖啡的化学成分，从而影响其生物效应。因此，本研究评估了脱咖啡因对阿拉比卡咖啡（Coffea arabica L.）样品中总酚和绿原酸水平的影响，以及摄入完整和脱咖啡因咖啡对正常和高脂血症大鼠脂质谱、止血和血液参数的影响。使用完整和脱咖啡因冻干咖啡（种植于巴西的阿拉比卡咖啡）样品进行化学分析（总酚、绿原酸和咖啡因含量）。对于生物测定，使用非冻干样品制备咖啡饮料（10%w/v），并通过灌胃（7.2 mL/kg/天）给予动物 30 天。在开始用咖啡饮料治疗 31 天后，用三油酸甘油酯（Triton WR-1339）（300 mg/kg 体重）诱导动物发生高脂血症。在第 32 天，抽取血液以确定脂质谱、血小板聚集、凝血酶原时间、部分激活的凝血酶原时间和血液学参数。完整咖啡饮料中两种酚类化合物和绿原酸的含量明显低于脱咖啡因咖啡饮料。用三油酸甘油酯处理的动物表现出混合性高脂血症。尽管脱咖啡因过程导致总酚和绿原酸的相对增加，但咖啡饮料无法改变研究动物的脂质谱或止血和血液学参数。
