Karasakal Ayça, Ulu Sevgi Tatar
Department of Chemistry, Faculty of Science and Letters, Namik Kemal University, Tekirdag, Turkey.
Luminescence. 2014 May;29(3):284-7. doi: 10.1002/bio.2541. Epub 2013 Jun 19.
A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397 nm (excitation)/514 nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250 ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17 ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine.
建立了一种选择性和灵敏的荧光分光光度法用于测定人血浆和尿液中的阿莫沙平，并对该方法进行了验证。所建立的方法基于用5-二甲基氨基萘-1-磺酰氯（丹磺酰氯）进行标记，并在397nm（激发）/514nm（发射）处进行监测。该方法针对线性、检测限（LOD）、定量限（LOQ）、精密度、准确度、回收率和稳健性进行了验证。血浆和尿液的校准曲线分别在250-2500和50-1250 ng/mL的浓度范围内呈线性。血浆和尿液的LOD值分别计算为13.31和13.17 ng/mL。将所提出的方法应用于人血浆和尿液中阿莫沙平的研究。
