Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Chembiochem. 2013 Jul 22;14(11):1289-90. doi: 10.1002/cbic.201300342. Epub 2013 Jun 18.
Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.
标记化学物质的“单个”结果：本文提出了一种一步法、非酶促的 5hmC 巯基化方法，该方法由亚硫酸氢盐介导。5hmC 碱基可以在单链 DNA 中进行化学标记，并通过纳米孔测序进行检测。
