去糖基化/去唾液酸化的含 GcMAF 的人血清诱导巨噬细胞吞噬活性和体内抗肿瘤活性。
Degalactosylated/desialylated human serum containing GcMAF induces macrophage phagocytic activity and in vivo antitumor activity.
机构信息
Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima, Tokushima, Japan.
出版信息
Anticancer Res. 2013 Jul;33(7):2881-5.
BACKGROUND
The group-specific component protein-derived macrophage-activating factor (GcMAF) has various biological activities, such as macrophage activation and antitumor activity. Clinical trials of GcMAF have been carried out for metastatic breast cancer, prostate cancer, and metastatic colorectal cancer. In this study, despite the complicated purification process of GcMAF, we used enzymatically-treated human serum containing GcMAF with a considerable macrophage-stimulating activity and antitumor activity.
RESULTS
We detected GcMAF in degalactosylated/desialylated human serum by western blotting using an anti-human Gc globulin antibody, and Helix pomatia agglutinin lectin. We also found that GcMAF-containing human serum significantly enhanced the phagocytic activity of mouse peritoneal macrophages and extended the survival time of mice bearing Ehrlich ascites tumors.
CONCLUSION
We demonstrated that GcMAF-containing human serum can be used as a potential macrophage activator for cancer immunotherapy.
背景
特异性组份蛋白衍生的巨噬细胞激活因子（GcMAF）具有多种生物学活性，如激活巨噬细胞和抗肿瘤活性。GcMAF 的临床试验已用于转移性乳腺癌、前列腺癌和转移性结直肠癌。在这项研究中，尽管 GcMAF 的纯化过程很复杂，但我们使用了酶处理的含有相当巨噬细胞刺激活性和抗肿瘤活性的人血清 GcMAF。
结果
我们使用抗人 Gc 球蛋白抗体和 Helix pomatia 凝集素通过 Western blot 在去半乳糖基化/去唾液酸化的人血清中检测到 GcMAF。我们还发现，含 GcMAF 的人血清显著增强了小鼠腹腔巨噬细胞的吞噬活性，并延长了携带艾氏腹水瘤的小鼠的存活时间。
结论
我们证明了含 GcMAF 的人血清可作为癌症免疫治疗的潜在巨噬细胞激活剂。
Zotero 插件