Department of Medical Technology, School of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Anticancer Res. 2013 Jul;33(7):2895-900.
In tumors, monocytes differentiate into tumor-associated macrophages following interaction with cancer cells. We have previously reported that angiogenesis- and chemotaxis-related factors are associated with human monocyte differentiation following interaction with colon cancer cells. However, the exact nature of factors remains unknown. We investigated factors associated with differentiation of human colon cancer cells following interaction with monocytes.
The human colon cancer cell line DLD-1 was co-cultured with the human monocyte cell line THP-1. mRNA expression was analyzed by quantitative real-time PCR.
Expression of interleukin-1β, matrix metalloproteinase (MMP)-1, MMP-2, and MMP-3 increased in human colon cancer cells after co-culture with monocytes. Conversely, the expression of monocyte chemotactic protein-1, tumor necrosis factor-α, and signal transducer and activator of transcription-3 did not increase.
Differentiation of human colon cancer cells following interaction with monocytes may be associated with angiogenesis and metastasis but not chemotaxis and signaling pathways. Thus, angiogenesis- and metastasis-related factors associated with differentiation of human colon cancer cells may constitute important targets for colon cancer therapy.
在肿瘤中，单核细胞与癌细胞相互作用后分化为肿瘤相关巨噬细胞。我们之前报道过，与结肠癌细胞相互作用后，与血管生成和趋化相关的因子与人类单核细胞分化有关。然而，确切的因子性质仍不清楚。我们研究了与人类结肠癌细胞在与单核细胞相互作用后分化相关的因子。
将人结肠癌细胞系 DLD-1 与人类单核细胞系 THP-1 共培养。通过实时定量 PCR 分析 mRNA 表达。
与单核细胞共培养后，人结肠癌细胞中白细胞介素 1β、基质金属蛋白酶（MMP）-1、MMP-2 和 MMP-3 的表达增加。相反，单核细胞趋化蛋白 1、肿瘤坏死因子-α 和信号转导和转录激活因子 3 的表达没有增加。
与单核细胞相互作用后人类结肠癌细胞的分化可能与血管生成和转移有关，但与趋化作用和信号通路无关。因此，与人类结肠癌细胞分化相关的血管生成和转移相关因子可能成为结肠癌治疗的重要靶点。
