Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.
Oncol Rep. 2013 Sep;30(3):1449-54. doi: 10.3892/or.2013.2558. Epub 2013 Jun 20.
Cancer research is currently focused on blocking the metastatic process at its early steps. Some particularly attractive targets are metastasis suppressor genes, which control cancer cell dissemination. The aim of this study was to clarify the relationship between the expression of KiSS1, a metastasis suppressor gene, and disease progression in colorectal cancer patients. One-hundred and seventy-five patients who underwent surgery for colorectal cancer were enrolled in this study. We analyzed KiSS1 mRNA expression by real-time reverse transcription PCR in colorectal cancer tissue and paired adjacent normal mucosa. KiSS1 protein expression in early- and advanced-stage colorectal cancer samples was determined by immunohistochemical analysis. Decreased KiSS1 expression was significantly associated with lymph node metastasis and was an independent prognostic factor. Logistic regression analysis revealed that decreased KiSS1 expression was an independent risk factor for lymph node metastasis. Immunohistochemical analysis indicated that KiSS1 was highly expressed in the cell cytoplasm of early-stage colorectal cancer cells. The loss of KiSS1 appears to correlate with the progression of lymph node metastasis. An assessment of KiSS1 expression may assist in the accurate colorectal cancer diagnosis and may contribute to predict clinical outcomes.
癌症研究目前集中在阻断转移过程的早期步骤。一些特别有吸引力的靶点是转移抑制基因，它们控制癌细胞的扩散。本研究的目的是阐明在结直肠癌患者中，转移抑制基因 KiSS1 的表达与疾病进展之间的关系。本研究纳入了 175 名接受结直肠癌手术的患者。我们通过实时逆转录聚合酶链反应分析结直肠癌组织和配对的相邻正常黏膜中的 KiSS1 mRNA 表达。通过免疫组织化学分析确定早期和晚期结直肠癌样本中的 KiSS1 蛋白表达。KiSS1 表达降低与淋巴结转移显著相关，是独立的预后因素。逻辑回归分析表明，KiSS1 表达降低是淋巴结转移的独立危险因素。免疫组织化学分析表明，KiSS1 在早期结直肠癌细胞的细胞质中高表达。KiSS1 的丢失似乎与淋巴结转移的进展相关。评估 KiSS1 的表达可能有助于结直肠癌的准确诊断，并有助于预测临床结局。
