Lazarus D, Yamin M, McCarthy K, Schneeberger E E, Kradin R
Department of Pathology, Massachusetts General Hospital, Boston 02114.
Am J Respir Cell Mol Biol. 1990 Aug;3(2):103-11. doi: 10.1165/ajrcmb/3.2.103.
Anti-RMA is a murine anti-rat monoclonal antibody that binds to a 120-kD surface membrane antigen expressed primarily by alveolar macrophages. Saline-lavaged alveolar macrophages (AM) formed clusters after incubation with anti-RMA. Anti-RMA produced multinucleated giant cells (MGC) in approximately 15% of adherent AM, and the F (ab')2 fragment of anti-RMA yielded MGC in approximately 9% of AM. The Fab fragment of anti-RMA did not promote MGC formation, nor did the murine anti-rat monoclonal antibodies OX41 and W3/25 (anti-CD4). Although anti-RMA produced a tenfold increase in [3H]thymidine incorporation by AM, it yielded a minimal increase in the number of AM. Autoradiography of AM stimulated with anti-RMA showed heterogeneous labeling of nuclei in MGC, suggesting that 3H-labeled AM may fuse with AM that are not actively synthesizing DNA. These findings suggest that binding of anti-RMA to AM may activate DNA synthesis, and promote clustering and fusion of AM, leading to MGC formation.
抗RMA是一种鼠抗大鼠单克隆抗体，它能与主要由肺泡巨噬细胞表达的120-kD表面膜抗原结合。用生理盐水灌洗得到的肺泡巨噬细胞（AM）与抗RMA孵育后形成了细胞簇。抗RMA在大约15%的贴壁AM中产生了多核巨细胞（MGC），抗RMA的F（ab'）2片段在大约9%的AM中产生了MGC。抗RMA的Fab片段没有促进MGC的形成，鼠抗大鼠单克隆抗体OX41和W3/25（抗CD4）也没有。尽管抗RMA使AM的[3H]胸苷掺入量增加了10倍，但它使AM的数量仅有少量增加。用抗RMA刺激后的AM的放射自显影显示MGC中的细胞核有不均匀的标记，这表明3H标记的AM可能与未活跃合成DNA的AM融合。这些发现表明抗RMA与AM的结合可能激活DNA合成，并促进AM的聚集和融合，从而导致MGC的形成。
