Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
Cytometry A. 2013 Aug;83(8):692-701. doi: 10.1002/cyto.a.22317. Epub 2013 Jun 20.
The cytometric enumeration and characterization of antigen-specific lymphocytes, as introduced about 15 years ago, has contributed significantly to our understanding of adaptive immune responses in health and disease. Despite the development of several technologies, allowing to directly or indirectly analyze many aspects of lymphocyte specificity and function, several unresolved issues remain, due to the low frequency of certain antigen-specific lymphocyte subsets and the complexity of T cell antigen recognition. This is especially true for CD4(+) conventional as well as regulatory T cells, which bring major contributions to immune protection and pathology. Here we review the current technologies for the analysis of antigen specific T cells within the physiologic T cell repertoire and with a special focus on recent technologies addressing the analysis of rare antigen-specific T cell populations including naive and regulatory T cells.
约 15 年前引入的细胞计量术对特异性淋巴细胞的计数和鉴定，极大地促进了我们对健康和疾病中的适应性免疫反应的理解。尽管已经开发出几种技术，能够直接或间接地分析淋巴细胞特异性和功能的许多方面，但由于某些抗原特异性淋巴细胞亚群的频率较低以及 T 细胞抗原识别的复杂性，仍存在一些尚未解决的问题。这在 CD4(+)常规 T 细胞和调节性 T 细胞中尤其如此，它们为免疫保护和病理学带来了重大贡献。在这里，我们回顾了分析生理 T 细胞库中抗原特异性 T 细胞的当前技术，并特别关注最近用于分析包括幼稚和调节性 T 细胞在内的稀有抗原特异性 T 细胞群体的技术。
