测定莫西沙星和加替沙星在 BACTEC MGIT 960 系统中分枝杆菌药物敏感性试验的临界浓度。
Determination of critical concentrations of moxifloxacin and gatifloxacin for drug susceptibility testing of Mycobacterium tuberculosis in the BACTEC MGIT 960 system.
机构信息
Moscow Scientific and Clinical Antituberculosis Center of Moscow Government Health Department, Moscow, 107014 Stromynka 10, Russia.
出版信息
J Antimicrob Chemother. 2013 Oct;68(10):2274-81. doi: 10.1093/jac/dkt202. Epub 2013 Jun 19.
OBJECTIVES
To determine critical concentrations of moxifloxacin and gatifloxacin for rapid evaluation of drug susceptibility of Mycobacterium tuberculosis in Middlebrook 7H9 broth using the automated system BACTEC™ MGIT™ 960.
METHODS
In total we studied 152 strains. Critical concentrations of moxifloxacin and gatifloxacin in the BACTEC™ MGIT™ 960 and on Lowenstein-Jensen medium were determined using a panel of 68 strains. The proportion method on Middlebrook 7H10 medium was used as a reference method. Drug susceptibility testing against fluoroquinolones was done for the other 84 strains using the established critical concentrations. The gyrA and gyrB genes of all strains were studied by molecular tests.
RESULTS
The critical concentrations determined for moxifloxacin and gatifloxacin were 0.25 mg/L in the BACTEC™ MGIT™ 960 system and 0.75 mg/L on Lowenstein-Jensen medium (absolute concentration method).
CONCLUSIONS
The moxifloxacin and gatifloxacin critical concentrations that we have established are reliable for rapid drug susceptibility testing in the BACTEC™ MGIT™ 960.
目的
使用自动化系统 BACTEC™ MGIT™ 960 确定莫西沙星和加替沙星对结核分枝杆菌在 Middlebrook 7H9 肉汤中快速药敏评估的临界浓度。
方法
我们共研究了 152 株菌株。使用 68 株菌株的面板确定 BACTEC™ MGIT™ 960 和 Lowenstein-Jensen 培养基中的莫西沙星和加替沙星临界浓度。比例法在 Middlebrook 7H10 培养基中用作参考方法。使用已建立的临界浓度对其他 84 株菌株进行针对氟喹诺酮类药物的药敏试验。对所有菌株的 gyrA 和 gyrB 基因进行分子检测。
结果
在 BACTEC™ MGIT™ 960 系统中确定的莫西沙星和加替沙星的临界浓度分别为 0.25 mg/L 和 Lowenstein-Jensen 培养基中的 0.75 mg/L（绝对浓度法）。
结论
我们确定的莫西沙星和加替沙星的临界浓度可用于 BACTEC™ MGIT™ 960 中的快速药敏试验。
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