Olszewska Ewa, Borzym-Kluczyk Malgorzata, Rzewnicki Ireneusz, Wojtowicz Jerzy, Rogowski Marek, Pietruski Jan Krzysztof, Czajkowska Aneta, Sieskiewicz Andrzej
Department of Otolaryngology, Medical University of Bialystok, Poland.
Contemp Oncol (Pozn). 2012;16(2):154-8. doi: 10.5114/wo.2012.28795. Epub 2012 May 29.
Lysosomal exoglycosidases, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequence-based families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx cancer compared to controls.
Larynx cancer (n = 21) and normal healthy tissue (n = 21) were collected from patients during total laryngectomy. A biopsy of macroscopically healthy tissue in the area of the lower 1/3 of omohyoid muscle was taken for frozen sections in each case and these served as controls. The release of p-nitrophenol from p-nitrophenol derivatives of MAN and GAL was used.
In all specimens we observed significantly higher activity of investigated enzymes in larynx cancer compared with controls. The mean release of MAN from activated cells was 3.702 ±1.3245 nkat/g wet tissue compared to controls (1.614 ±0.8220 nkat/g wet tissue). The mean release of GAL from the activated cells was 3.383 ±2.1980 nkat/g wet tissue compared to controls (2.137 ±1.3685 nkat/g wet tissue). Differences in observed activity were statistically significant.
The present data indicate that MAN and GAL are significantly and consistently elevated in larynx cancer growth. It also means that catabolic reactions involving glycoproteins, glycolipids and proteoglycans may play a role in larynx cancer. Further research should also evaluate the relative importance of these particular exoglycosidases in indicating the progress of the disease in considering the spectrum of identified marker mediators.
溶酶体外切糖苷酶，如α-甘露糖苷酶（MAN）和β-半乳糖苷酶（GAL），存在于不同的基于糖苷水解酶序列的家族中。大量研究已证实MAN发挥的作用，其在杂合N-聚糖的修饰和多样化过程中起关键作用，这些过程与癌症存在紧密的细胞联系。因此，本研究旨在探讨与对照组相比，喉癌中MAN和GAL的活性。
在全喉切除术期间从患者身上收集喉癌组织（n = 21）和正常健康组织（n = 21）。在每种情况下，取肩胛舌骨肌下1/3区域宏观健康组织的活检样本用于制作冰冻切片，这些样本用作对照。采用从MAN和GAL的对硝基苯酚衍生物中释放对硝基苯酚的方法。
在所有标本中，我们观察到与对照组相比，喉癌中所研究酶的活性显著更高。与对照组（1.614±0.8220 nkat/g湿组织）相比，激活细胞中MAN的平均释放量为3.702±1.3245 nkat/g湿组织。与对照组（2.137±1.3685 nkat/g湿组织）相比，激活细胞中GAL的平均释放量为3.383±2.1980 nkat/g湿组织。观察到的活性差异具有统计学意义。
目前的数据表明，MAN和GAL在喉癌生长过程中显著且持续升高。这也意味着涉及糖蛋白、糖脂和蛋白聚糖的分解代谢反应可能在喉癌中起作用。在考虑已鉴定的标志物介质谱时，进一步的研究还应评估这些特定外切糖苷酶在指示疾病进展方面的相对重要性。
