Department of Biotechnology, Bells University of Technology, Ota, Nigeria.
Asian Pac J Trop Med. 2013 Aug;6(8):589-94. doi: 10.1016/S1995-7645(13)60102-9.
To characterize the genetic diversity of Plasmodium falciparum (P. falciparum) field isolates in children from Lafia, North-central Nigeria, using the highly polymorphic P. falciparum merozoite surface protein 2 (MSP-2) gene as molecular marker.
Three hundred and twenty children were enrolled into the study between 2005 and 2006. These included 140 children who presented with uncomplicated malaria at the Dalhatu Araf Specialist Hospital, Lafia and another 180 children from the study area with asymptomatic infection. DNA was extracted from blood spot on filter paper and MSP-2 genes were genotyped using allele-specific nested PCR in order to analyze the genetic diversity of parasite isolates.
A total of 31 and 34 distinct MSP-2 alleles were identified in the asymptomatic and uncomplicated malaria groups respectively. No difference was found between the multiplicity of infection in the asymptomatic group and that of the uncomplicated malaria group (P>0.05). However, isolates of the FC27 allele type were dominant in the asymptomatic group whereas isolates of the 3D7 allele type were dominant in the uncomplicated malaria group.
This study showed a high genetic diversity of P. falciparum isolates in North-central Nigeria and is comparable to reports from similar areas with high malaria transmission intensity.
利用高度多态的恶性疟原虫裂殖子表面蛋白 2（MSP-2）基因作为分子标记，对尼日利亚中北部拉菲亚儿童中的恶性疟原虫（P. falciparum）现场分离株的遗传多样性进行特征描述。
2005 年至 2006 年期间，共有 320 名儿童入组研究。其中包括 140 名在拉菲亚 Dalhatu Araf 专科医院就诊的无并发症疟疾儿童，以及来自研究区域的另外 180 名无症状感染儿童。从滤纸上的血斑中提取 DNA，并使用等位基因特异性嵌套 PCR 对 MSP-2 基因进行基因分型，以分析寄生虫分离株的遗传多样性。
在无症状组和无并发症疟疾组中分别鉴定出 31 种和 34 种不同的 MSP-2 等位基因。无症状组和无并发症疟疾组之间的感染多样性没有差异（P＞0.05）。然而，FC27 等位基因类型的分离株在无症状组中占优势，而 3D7 等位基因类型的分离株在无并发症疟疾组中占优势。
本研究显示尼日利亚中北部的恶性疟原虫分离株具有很高的遗传多样性，与高疟疾传播强度的类似地区的报告相当。
