Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea.
Food Chem. 2013 Nov 15;141(2):695-701. doi: 10.1016/j.foodchem.2013.04.059. Epub 2013 Apr 29.
In polymeric nanoparticle preparation, despite similar conditions, large fluctuations in particle size distributions are usually observed. Herein, we demonstrate that the intermittent addition of a desolvating agent can improve reproducibility in the preparation of polymeric bovine serum albumin (BSA) nanoparticles. Using this modification, BSA nanoparticles of controlled size can be manufactured with narrow particle size distributions. In our study, ethanol as a desolvating agent was added intermittently to 1% BSA solutions at different pHs with stirring at 700rpm. The effect of the preparation parameters on size and optical density of the fabricated nanoparticles were studied. The average particles sizes of BSA nanoparticles prepared at pH values of 6, 7 and 9 were approximately 100, 200 and 300nm, respectively. As ethanol addition increased, desolvation of BSA molecules resulted in formation of loose-structured particles with pH-dependent size. Beyond that, only particle density increased, but size remained unchanged with further addition of ethanol. Consistently uniform particle size distribution was achieved by adding ethanol intermittently.
在聚合物纳米粒子的制备中，尽管条件相似，但通常会观察到粒径分布的大幅波动。在此，我们证明了间歇加入去溶剂化剂可以改善聚合物牛血清白蛋白（BSA）纳米粒子制备的重现性。通过这种改进，可以制造具有窄粒径分布的、尺寸可控的 BSA 纳米粒子。在我们的研究中，将乙醇作为去溶剂化剂间歇加入到不同 pH 值的 1% BSA 溶液中，在 700rpm 下搅拌。研究了制备参数对所制备纳米粒子的尺寸和光密度的影响。在 pH 值为 6、7 和 9 下制备的 BSA 纳米粒子的平均粒径分别约为 100、200 和 300nm。随着乙醇添加量的增加，BSA 分子的去溶剂化导致形成具有 pH 依赖性的疏松结构颗粒。此外，随着乙醇进一步添加，只有颗粒密度增加，而粒径保持不变。通过间歇添加乙醇可以实现一致均匀的颗粒尺寸分布。
