Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
Food Chem. 2013 Nov 15;141(2):1008-18. doi: 10.1016/j.foodchem.2013.04.050. Epub 2013 Apr 24.
This study was to investigate the anticancer effects of the peel polyphenolic extract (HPP) and flesh polyphenolic extract (HFP) from hawthorn fruit in human MCF-7 breast cancer cells. It was found that the polyphenol and flavonoid contents of HPP were significant higher than that of HFP. Both HPP and HFP inhibited cell growth in a dose-dependent manner with the IC50 of 88.6μg/mL and 175.5μg/mL, respectively, suggesting that HPP was more effective against MCF-7 cells than HFP. Flow cytometric analysis revealed that both HPP and HFP mediated the cell-cycle arrest at the S-phase, and also dose-dependently led to apoptosis of MCF-7 cells via the mitochondrial pathway, as evidenced by the activation of caspase-3 and caspase-9 and the elevation of intracellular ROS production. All these findings indicate that hawthorn fruit, especially its peel, is an excellent source of natural chemopreventive agents in the treatment of breast cancer.
本研究旨在探讨山楂果实的果皮多酚提取物（HPP）和果肉多酚提取物（HFP）对人 MCF-7 乳腺癌细胞的抗癌作用。结果发现，HPP 的多酚和类黄酮含量明显高于 HFP。HPP 和 HFP 均呈剂量依赖性抑制细胞生长，IC50 分别为 88.6μg/mL 和 175.5μg/mL，表明 HPP 对 MCF-7 细胞的抑制作用强于 HFP。流式细胞术分析显示，HPP 和 HFP 均能使细胞周期阻滞在 S 期，并通过线粒体途径诱导 MCF-7 细胞凋亡，这可通过 caspase-3 和 caspase-9 的激活以及细胞内 ROS 生成的增加来证明。所有这些发现表明，山楂果实，特别是其果皮，是治疗乳腺癌的天然化学预防剂的良好来源。
