School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
FEBS Lett. 2013 Aug 2;587(15):2441-7. doi: 10.1016/j.febslet.2013.06.014. Epub 2013 Jun 19.
Genome-wide Okazaki fragment distribution can differentiate the discontinuous from the semi-discontinuous DNA replication model. Here, we investigated the genome-wide Okazaki fragment distribution in Saccharomyces cerevisiae S288C. We improved the method based upon lambda exonuclease digestion to purify Okazaki fragments from S288C yeast cells, followed by Illumina sequencing. The distribution of Okazaki fragments around confirmed replication origins, including two highly efficient replication origins, supported the discontinuous DNA replication model. In S. cerevisiae mitochondria, Okazaki fragments were overrepresented in the transcribed regions, indicating the interplay between transcription and DNA replication.
全基因组冈崎片段分布可区分不连续和半不连续 DNA 复制模型。在这里，我们研究了酿酒酵母 S288C 的全基因组冈崎片段分布。我们改进了基于λ核酸外切酶消化的方法，从 S288C 酵母细胞中纯化冈崎片段，然后进行 Illumina 测序。冈崎片段在已确认的复制起点周围的分布，包括两个高效复制起点，支持不连续 DNA 复制模型。在酿酒酵母线粒体中，冈崎片段在转录区域中过度表达，表明转录和 DNA 复制之间的相互作用。
