探寻多细胞真核生物中DNA复制的起源

Urban John M, Foulk Michael S, Casella Cinzia, Gerbi Susan A

Division of Biology and Medicine, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University Sidney Frank Hall, 185 Meeting Street, Providence, RI 02912 USA.

Division of Biology and Medicine, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University Sidney Frank Hall, 185 Meeting Street, Providence, RI 02912 USA ; Department of Biology, Mercyhurst University 501 East 38th Street, Erie, PA 16546 USA.

F1000Prime Rep. 2015 Mar 3;7:30. doi: 10.12703/P7-30. eCollection 2015.

Origins of DNA replication (ORIs) occur at defined regions in the genome. Although DNA sequence defines the position of ORIs in budding yeast, the factors for ORI specification remain elusive in metazoa. Several methods have been used recently to map ORIs in metazoan genomes with the hope that features for ORI specification might emerge. These methods are reviewed here with analysis of their advantages and shortcomings. The various factors that may influence ORI selection for initiation of DNA replication are discussed.

DNA复制起点（ORIs）出现在基因组中的特定区域。虽然DNA序列决定了芽殖酵母中ORIs的位置，但后生动物中确定ORI的因素仍然不清楚。最近已经使用了几种方法来绘制后生动物基因组中的ORIs，希望能找出确定ORI的特征。本文对这些方法进行了综述，并分析了它们的优缺点。还讨论了可能影响DNA复制起始时ORI选择的各种因素。