Ramasamy R, Mota de Freitas D, Bansal V K, Dorus E, Labotka R J
Department of Chemistry, Loyola University of Chicago, IL 60626.
Clin Chim Acta. 1990 Apr 30;188(2):169-76. doi: 10.1016/0009-8981(90)90161-k.
We have applied a nuclear magnetic resonance (NMR) method, based on the 7Li nucleus, to discriminate between intracellular and extracellular lithium ions (Li+) in red blood cell (RBC) suspensions. The NMR method was compared with atomic absorption, a technique that requires physical separation of intra- and extracellular Li+ prior to chemical analysis. The rates and rate constants of RBC Na(+)-Li+ countertransport measured by the 7Li NMR method correlated significantly with the measurements made by atomic absorption for both the hypertensive (r = 0.964) and control (r = 0.961) groups. The rates of RBC Na(+)-Li+ countertransport measured by NMR were significantly higher for hypertensive patients than for normotensive controls. The fact that the NMR method does not require cell membrane lysis, and its potential to reveal structural and mechanistic information on Li+ binding and transport in cellular systems, makes it promising for understanding the basis of Li+ transport variations in RBCs, and possibly other tissues, from hypertensive patients.
我们应用了一种基于锂 - 7原子核的核磁共振（NMR）方法，来区分红细胞（RBC）悬液中的细胞内和细胞外锂离子（Li +）。将该核磁共振方法与原子吸收法进行了比较，原子吸收法是一种在化学分析之前需要对细胞内和细胞外Li +进行物理分离的技术。通过锂 - 7核磁共振方法测得的红细胞钠（+）-锂（+）逆向转运速率和速率常数，与高血压组（r = 0.964）和对照组（r = 0.961）通过原子吸收法测得的结果显著相关。通过核磁共振测得的高血压患者红细胞钠（+）-锂（+）逆向转运速率明显高于血压正常的对照组。核磁共振方法不需要细胞膜裂解，并且有潜力揭示细胞系统中Li +结合和转运的结构和机制信息，这使得它有望用于理解高血压患者红细胞以及可能其他组织中Li +转运变化的基础。
