丁酸甘油酯作为测定牛奶中脂肪酶活性的底物。
Tributyrate as a substrate for the determination of lipase activity in milk.
作者信息
Castberg H B, Solberg P
出版信息
J Dairy Res. 1975 Jun;42(2):247-53. doi: 10.1017/s0022029900015284.
PMID:237940
Abstract
A procedure is described for the determination of the lipolytic activity in milk using tributyrate as substrate. The method is based on the titration of liberated butyric acid in a diethyl ether: light petroleum (2-75:1, v/v) extract of the incubation mixture. Some of the fundamental factors involved in the procedure are discussed.
摘要
描述了一种以丁酸甘油酯为底物测定牛奶中脂解活性的方法。该方法基于对孵育混合物的乙醚：轻质石油（2 - 75:1，v/v）提取物中释放的丁酸进行滴定。讨论了该方法中涉及的一些基本因素。
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