Sen S, Erba E, D'Incalci M
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Cytometry. 1990;11(5):595-602. doi: 10.1002/cyto.990110506.
U937 human histiocytic lymphoma cell line and SW626 ovarian carcinoma line of human origin were synchronised using very low, nontoxic concentrations (0.04-0.08 microM for 16-24 h) of methotrexate (MTX) under standard culture conditions. Satisfactory synchrony was achieved to study S phase events. Various kinetic behaviours and biological properties of the synchronised cells are considered for characterisation of the system. MTX-synchronisation was compared with that induced by aphidicolin (APC) alone and by serum deprivation and APC. In some cancer cell lines MTX appears to be the best choice for obtaining highly synchronised cell populations without cytotoxicity or physiological perturbations.
使用极低的无毒浓度（0.04 - 0.08微摩尔，作用16 - 24小时）的甲氨蝶呤（MTX）在标准培养条件下将人源U937组织细胞淋巴瘤细胞系和SW626卵巢癌细胞系同步化。为研究S期事件实现了令人满意的同步化。考虑同步化细胞的各种动力学行为和生物学特性以表征该系统。将MTX同步化与单独使用阿非科林（APC）以及血清饥饿和APC诱导的同步化进行了比较。在一些癌细胞系中，MTX似乎是获得高度同步化细胞群体而无细胞毒性或生理扰动的最佳选择。
