Hemmer J
Department of Oral and Maxillofacial Surgery, University of Ulm, Federal Republic of Germany.
Cytometry. 1990;11(5):603-9. doi: 10.1002/cyto.990110507.
In vitro bromodeoxyuridine (BrdUrd) incubated single-cell suspensions obtained from solid tumors were fixed on slides for subsequent sample processing. As dispersal of nuclei largely was avoided, only small amounts of cells were needed for examination. The sensitivity of detecting even low BrdUrd incorporation rates could be improved by treatment with intense DNA denaturation conditions. This technique was applied to monitor cytokinetic response to chemotherapy and radiation in oral carcinomas by analysing biopsies taken consecutively in the course of treatment. By combining BrdUrd labeling and DNA flow cytometry, cells arrested in S phase easily could be distinguished from cells showing continuous proliferation.
将从实体瘤中获得的体外溴脱氧尿苷（BrdUrd）孵育单细胞悬液固定在载玻片上，用于后续样本处理。由于很大程度上避免了细胞核的分散，检查仅需少量细胞。通过强烈的DNA变性条件处理，即使检测低BrdUrd掺入率的灵敏度也能得到提高。该技术通过分析治疗过程中连续采集的活检样本，应用于监测口腔癌对化疗和放疗的细胞动力学反应。通过结合BrdUrd标记和DNA流式细胞术，容易区分停滞在S期的细胞和显示持续增殖的细胞。
