Jourdi Hussam, Nawa Hiroyuki
Department of Molecular Neurobiology, Brain Research Institute, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Acta Med Biol (Niigata). 2002;50(3):107-115.
Different cytokines and growth factors, together with their receptors, are expressed in brain tissue. One such molecule is the basic fibroblast growth factor (bFGF) that has recently been shown to promote survival following insults to neurons or . In this study, we found that repeated treatment of neocortical cultures with bFGF modulated the expression of various PDZ domain-containing proteins (SAP97, GRIP1, Pick1, and PSD-93) and that the patterns of their immunostaining matched the bFGF effects on their total protein expression. For instance, bFGF decreased the expression of SAP97, GRIP1, and Pick1 (PDZ proteins that interact with the AMPA-type glutamate receptor subunits GluR1 and GluR2/3). PSD-93, which associates with the NMDA-type glutamate receptor, was increased by bFGF. Moreover, the interactions of GluR1 with SAP97 and GluR2 with GRIP1 were down-regulated by the repeated bFGF stimulation, as revealed by co-immunoprecipitation. Together, these results describe a novel function of bFGF in the regulation of expression of PDZ proteins.
不同的细胞因子和生长因子及其受体在脑组织中表达。其中一种分子是碱性成纤维细胞生长因子（bFGF），最近的研究表明，它能促进神经元在遭受损伤后的存活。在本研究中，我们发现用bFGF反复处理新皮质培养物可调节多种含PDZ结构域蛋白（SAP97、GRIP1、Pick1和PSD - 93）的表达，并且它们的免疫染色模式与bFGF对其总蛋白表达的影响相匹配。例如，bFGF降低了SAP97、GRIP1和Pick1（与AMPA型谷氨酸受体亚基GluR1和GluR2/3相互作用的PDZ蛋白）的表达。与NMDA型谷氨酸受体相关的PSD - 93则因bFGF而增加。此外，共免疫沉淀结果显示，反复的bFGF刺激下调了GluR1与SAP97以及GluR2与GRIP1之间的相互作用。这些结果共同描述了bFGF在调节PDZ蛋白表达方面的新功能。
