Iakubovskaia R I, Kazachkina N I, Karmakova T A, Nemtsova E R, Korosteleva M D, Aleksander S K, Avdeev G I, Sorkin E E, Baryshnikov A Iu, Korotkova O V
Eksp Onkol. 1990;12(4):61-5.
The glycoprotein with the molecular weight of 82,500 D (Ag3) was extracted from the native membranes of fatty globules of human milk and was purified by isoelectrofocusing. Polyclonal antisera (PCA) as well as monoclonal antibodies (MoAb)-ICO-21, ICO-22, ICO-26, ICO-29 against this membrane antigen were produced. The localization of the antigen in the human normal and malignant tissues was tested by the immunochemical technique. Apical staining of the Ag3 was in the acinus and duct cells of the normal breast. Weak staining with the antibodies to Ag3 was revealed in the epithelium cells of ovaries, bronchi, kidney tubules and uterine tubes. The far more pronounced staining of the Ag3 with PCA and MCA was observed in the cell cytoplasma both in the primary and metastatic breast and ovarian tumours. The specificity of the MoAb to Ags suggests that they will be useful in the diagnosis of the malignant disease of the breast and ovary.
从人乳脂肪球的天然膜中提取分子量为82,500道尔顿的糖蛋白（Ag3），并通过等电聚焦进行纯化。制备了针对该膜抗原的多克隆抗血清（PCA）以及单克隆抗体（MoAb）-ICO-21、ICO-22、ICO-26、ICO-29。采用免疫化学技术检测该抗原在人正常组织和恶性组织中的定位。Ag3在正常乳腺的腺泡和导管细胞中呈顶端染色。在卵巢、支气管、肾小管和输卵管的上皮细胞中，用抗Ag3抗体显示出弱阳性染色。在原发性和转移性乳腺及卵巢肿瘤的细胞质中，用PCA和MCA观察到Ag3染色更为明显。MoAb对Ag的特异性表明它们将有助于乳腺和卵巢恶性疾病的诊断。
