Boswell H S, Mochizuki D Y, Burgess G S, Gillis S, Walker E B, Anderson D, Williams D E
Hematology/Oncology Division, Veterans Administration Medical Center, Indianapolis, Indiana.
Exp Hematol. 1990 Aug;18(7):794-800.
A clonal marrow-adherent stromal cell line, +/+-1 LDA11, was derived and found to produce hemopoietic stimulatory activity for an interleukin 3 (IL-3)-dependent mast cell line, NFS/N1. This factor-dependent mast cell line displayed restricted growth factor responsiveness to only IL-3, interleukin 4 (IL-4), and the stromal cell-produced factor. The factor produced by stromal cells was distinguished from IL-3 and IL-4 and was characterized biochemically. This factor appears to be a novel mast cell growth factor (MCGF-3) capable of synergizing with IL-3 and IL-4. It may have broader reactivity in hemopoiesis than simply IL-3-dependent mast cells, and it may prove relevant to stromal cell-mediated hemopoiesis.
一种克隆的骨髓黏附基质细胞系，即 +/+-1 LDA11，已被分离出来，并发现它能为依赖白细胞介素 3（IL-3）的肥大细胞系 NFS/N1 产生造血刺激活性。这种依赖因子的肥大细胞系对生长因子的反应具有局限性，仅对 IL-3、白细胞介素 4（IL-4）和基质细胞产生的因子有反应。基质细胞产生的因子与 IL-3 和 IL-4 不同，并对其进行了生化特性鉴定。该因子似乎是一种新型的肥大细胞生长因子（MCGF-3），能够与 IL-3 和 IL-4 协同作用。它在造血过程中的反应可能比单纯依赖 IL-3 的肥大细胞更广泛，并且可能与基质细胞介导的造血作用相关。
