Laktionov P P, Maksimov D A, Andreeva E N, Shloma V V, Beliakin S N
Tsitologiia. 2013;55(3):185-9.
Significant progress in the developmental biology of Drosophila is largely due to the improvement of methods of genetic manipulation and, in particular, development of ways to create mosaic organisms. The main characteristic of the mosaic organisms is the presence of genetically different populations of cells. For example, some tissues express a transgenic reporter gene that is absent in other cells of the body. This principle is used in a variety of the methods with the common name lineage tracing. The essence of these approaches is to perform the targeted changes in the genetic apparatus of progenitor cells that give rise to cell lines or organs and tissues. Genetic modification in progenitor cells, such as the ability to express a fluorescent protein, will be inherited by the next cell generations, and, as a result, the entire cell line or tissue will have a tag, which distinguishes it from the rest of the body. The lineage tracing methods allow tracking the cell generations, studying the cell proliferation process, tracing their origin and investigating the function of genes of interest in the development of a single tissue or organ. We have designed an approach to selectively label germ line or somatic cells in the gonads of Drosophila.
果蝇发育生物学的重大进展很大程度上归功于基因操作方法的改进，特别是创造镶嵌体生物方法的发展。镶嵌体生物的主要特征是存在基因不同的细胞群体。例如，一些组织表达转基因报告基因，而身体的其他细胞中则不存在该基因。这一原理被用于各种统称为谱系追踪的方法中。这些方法的本质是对产生细胞系或器官及组织的祖细胞的遗传装置进行定向改变。祖细胞中的基因修饰，如表达荧光蛋白的能力，将被下一代细胞继承，结果，整个细胞系或组织将有一个标签，使其与身体的其他部分区分开来。谱系追踪方法允许追踪细胞世代，研究细胞增殖过程，追踪它们的起源，并研究感兴趣的基因在单个组织或器官发育中的功能。我们设计了一种方法来选择性地标记果蝇性腺中的生殖细胞或体细胞。
