Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Stn. Lennoxville, Sherbrooke, Quebec, Canada J1M 08C.
Bioresour Technol. 2013 Aug;142:663-71. doi: 10.1016/j.biortech.2013.05.089. Epub 2013 May 29.
Psychrophilic (20°C) specific methane (CH4) yield from cellulose (C), xylan (X), cellulose/xylan mixture (CX), cow feces (CF), and wheat straw (WS) achieved (Nl CH4 kg(-1)VS) of 338.5 ± 14.3 (C), 310.5 ± 3.4 (X), 305.5 ± 29.6 (CX mixture), and 235.3 ± 22.7 (WS) during 56 days, and 237.6 ± 17.7 (CF) during 70 days. These yields corresponded to COD recovery of 73.3 ± 3.1% (C)=69.1 ± 0.76% (X)=67.3 ± 5.8% (CX mixture)>52.9 ± 2.6% (CF)>46.5 ± 2.7% (WS). Cellulose-fed culture had a lower and statistically different initial CH4 production rate from those calculated for cultures fed X, CX mixture, CF and WS. It seemed that the presence of hemicellulose in complex substrate such as wheat straw and cow feces supported the higher initial CH4 rate compared to cellulose. Biomethanation of the pure and complex lignocellulosic substrates tested is feasible at psychrophilic conditions given that a well-adapted inoculum is used; however, hydrolysis was the rate limiting step.
在 20°C 时，从纤维素 (C)、木聚糖 (X)、纤维素/木聚糖混合物 (CX)、牛粪便 (CF) 和小麦秸秆 (WS) 中获得的嗜冷（20°C）特异性甲烷 (CH4) 产量（Nl CH4 kg(-1)VS）分别为 338.5 ± 14.3（C）、310.5 ± 3.4（X）、305.5 ± 29.6（CX 混合物）和 235.3 ± 22.7（WS），持续 56 天，70 天内分别为 237.6 ± 17.7（CF）。这些产率对应于 COD 回收率为 73.3 ± 3.1%（C）=69.1 ± 0.76%（X）=67.3 ± 5.8%（CX 混合物）>52.9 ± 2.6%（CF）>46.5 ± 2.7%（WS）。与木聚糖、CX 混合物、CF 和 WS 相比，以纤维素为食的培养物的初始 CH4 产生速率较低且具有统计学差异。似乎在小麦秸秆和牛粪便等复杂基质中存在半纤维素，这与纤维素相比支持了更高的初始 CH4 速率。在使用适应良好的接种物的情况下，在低温条件下可以对纯木质纤维素和复杂木质纤维素底物进行生物甲烷化；然而，水解是限速步骤。
