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高粱(双色高粱)中假定的微小RNA及其靶基因的计算机分析。

In silico analysis of putative miRNAs and their target genes in sorghum (Sorghum bicolor).

作者信息

Ram Gobind, Sharma Arun Dev

机构信息

PG Department of Biotechnology, Lyallpur Khalsa College, GT Road, Jallandhar 144001, Punjab, India.

出版信息

Int J Bioinform Res Appl. 2013;9(4):349-64. doi: 10.1504/IJBRA.2013.054694.

Abstract

MicroRNAs (miRNAs) are small endogenous genes regulators which regulate different processes underlying plant adaptation to abiotic stresses. To gain a deep understanding of role of miRNAs in plants, in the present study, we computationally analyzed different sorghum miRNAs in the drought-induced gene sequences. Homologous miRNA were identified using different plant miRNA databases. Using previously established genes databases, various targets of sorghum miRNAs were predicted viz: transcription factors, chaperonins, metabolic enzymes and other gene targets necessary for proper plant development. Analysis of cis-elements provides molecular evidence for the possible involvement of miRNAs in the process of abiotic stress tolerance in sorghum. Based on these results, it was suggested that miRNAs may play an important role in water stress tolerance.

摘要

微小RNA(miRNA)是小型内源性基因调控因子,可调控植物适应非生物胁迫的不同过程。为深入了解miRNA在植物中的作用,在本研究中,我们对干旱诱导基因序列中的不同高粱miRNA进行了计算分析。使用不同的植物miRNA数据库鉴定同源miRNA。利用先前建立的基因数据库,预测了高粱miRNA的各种靶标,即:转录因子、伴侣蛋白、代谢酶以及植物正常发育所需的其他基因靶标。顺式元件分析为miRNA可能参与高粱非生物胁迫耐受性过程提供了分子证据。基于这些结果,表明miRNA可能在水分胁迫耐受性中发挥重要作用。

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