Zhai Xiao-Feng, Jiang Cheng-Ai, Wu Guang-Xue, Guan Yun-Tao
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Huan Jing Ke Xue. 2013 Apr;34(4):1421-7.
Denitrification of wastewater is one of the important sources of nitrous oxide (N2O). In this study, denitrifies were acclimated in a sequencing batch reactor with methanol and nitrate (NO3(-) -N) as the electron donor and acceptor, respectively. N2O emission during denitrification was examined both in typical cycles and in batch experiments under conditions of different electron acceptors, carbon/nitrogen (C/N) ratios and initial nitrite (NO2(-)-N) concentrations. With methanol as the organic carbon, the N2O emission was high with NO2(-)-N as the electron acceptor and the N2O emission was low with NO3(-) -N as the electron acceptor. The C/N ratios affected the emission of N2O by affecting activities of denitrifiers, and both the activity of denitrifiers and the emission of N2O decreased with decreasing C/N ratios. The N2O emission increased with increasing initial NO2(-) -N concentrations, and a certain range of NO2(-) -N concentrations enhanced the activity of denitrifiers. The N2O emission could be correlated very well with initial NO2(-) -N concentrations.
废水反硝化是一氧化二氮（N₂O）的重要来源之一。在本研究中，反硝化菌在序批式反应器中进行驯化，分别以甲醇和硝酸盐（NO₃⁻-N）作为电子供体和受体。在典型周期以及不同电子受体、碳氮（C/N）比和初始亚硝酸盐（NO₂⁻-N）浓度条件下的批次实验中，均对反硝化过程中的N₂O排放进行了检测。以甲醇作为有机碳源时，以NO₂⁻-N作为电子受体时N₂O排放较高，以NO₃⁻-N作为电子受体时N₂O排放较低。C/N比通过影响反硝化菌的活性来影响N₂O的排放，随着C/N比降低，反硝化菌的活性和N₂O排放均降低。随着初始NO₂⁻-N浓度的增加，N₂O排放增加，一定范围的NO₂⁻-N浓度可增强反硝化菌的活性。N₂O排放与初始NO₂⁻-N浓度具有很好的相关性。
