钙视网膜蛋白免疫组织化学：一种用于诊断先天性巨结肠的新的经济高效且简便的方法。
Calretinin immunohistochemistry: A new cost-effective and easy method for diagnosis of Hirschsprung's disease.
作者信息
Kannaiyan Lavanya, Madabhushi Sujani, Malleboyina Ramani, Are Narendra Kumar, Reddy K Ramesh, Rao Bhuvaneshwar
机构信息
Department of Pediatric Surgery, Niloufer Hospital for Women and Children, Osmania Medical College, Hyderabad, India.
出版信息
J Indian Assoc Pediatr Surg. 2013 Apr;18(2):66-8. doi: 10.4103/0971-9261.109355.
AIM
To evaluate the efficacy of calretinin immunostaining in diagnosing Hirschsprung's disease (HD).
MATERIALS AND METHODS
Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination.
RESULTS
Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining.
CONCLUSIONS
Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available.
目的
评估钙视网膜蛋白免疫染色在诊断先天性巨结肠（HD）中的疗效。
材料与方法
在1年时间内（2010年7月至2011年6月）研究了60例病例。有36例全层活检标本和24例切除标本。在常规组织病理学检查后，对石蜡包埋块进行钙视网膜蛋白处理。
结果
在36例活检标本中，19例通过苏木精和伊红（H&E）染色较早诊断出HD。2例患者可见神经节细胞，排除了HD。15例存在诊断困境，使用了钙视网膜蛋白。3个标本中发现神经节细胞，5个标本中发现神经纤维。在所有24例切除标本中，钙视网膜蛋白与H&E染色结果相关。
结论
钙视网膜蛋白在解决HD可疑和不确定病例方面极其有用。在无法进行乙酰胆碱酯酶组织化学的中心，它可作为一种有价值的、性价比高的诊断辅助手段。
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