Rezaei Marzieh, Zarkesh-Esfahani Sayyed H
Department of Biology, Faculty of Sciences, University of Isfahan, Hezar Jerib Street, Isfahan, Postcode: 81746-73695, Iran.
J Res Med Sci. 2012 Jul;17(7):681-5.
Human growth hormone (hGH) is a single-chain polypeptide that participates in a wide range of biological functions such as metabolism of proteins, carbohydrates and lipids as well as in growth, development and immunity. Growth hormone deficiency in human occurs both in children and adults. The routine treatment for this condition is administration of recombinant human growth hormone (rhGH) made by prokaryotes. Since nonglycosylated human growth hormone is a biologically active protein, prokaryotic expression systems are preferred for its production.
Different strains of E.coli were transformed by plasmid containing human growth hormone gene and cultured in different conditions. After induction by IPTG, recombinant human growth hormone production was assessed using ELISA, dot blotting and western blotting techniques.
High levels of rhGH were produced using E.coli prokaryotic protein production system.
This simple and cost effective production process could be recruited for large scale production of rhGH.
人生长激素（hGH）是一种单链多肽，参与蛋白质、碳水化合物和脂质代谢等广泛的生物学功能，以及生长、发育和免疫过程。人类生长激素缺乏症在儿童和成人中均有发生。针对这种情况的常规治疗方法是给予原核生物生产的重组人生长激素（rhGH）。由于非糖基化人生长激素是一种生物活性蛋白，原核表达系统更适合用于其生产。
用含人生长激素基因的质粒转化不同菌株的大肠杆菌，并在不同条件下培养。经异丙基-β-D-硫代半乳糖苷（IPTG）诱导后，采用酶联免疫吸附测定（ELISA）、斑点印迹和蛋白质印迹技术评估重组人生长激素的产量。
利用大肠杆菌原核蛋白生产系统产生了高水平的rhGH。
这种简单且具有成本效益的生产工艺可用于大规模生产rhGH。
