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体外和裸鼠实验中 CD44+/CD24- 乳腺癌 MCF7 细胞侵袭和致瘤能力增强。

Increased invasion and tumorigenicity capacity of CD44+/CD24- breast cancer MCF7 cells in vitro and in nude mice.

机构信息

Department of Radiotherapy, Second Affiliated Hospital of Jilin University, 130021, Changchun, China.

出版信息

Cancer Cell Int. 2013 Jun 24;13(1):62. doi: 10.1186/1475-2867-13-62.

DOI:10.1186/1475-2867-13-62
PMID:23799994
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3717045/
Abstract

BACKGROUND

Identification of cancer stem cells (CSCs) and their behaviors will provide insightful information for the future control of human cancers. This study investigated CD44 and CD24 cell surface markers as breast cancer CSC markers in vitro and in vivo.

METHODS

Flow cytometry with CD44 and CD24 markers was used to sort breast cancer MCF7 cells for scanning electron microscopy (SEM), tumor cell invasion assay, and nude mouse xenograft assay.

RESULTS

Flow cytometry assay using CD44 and CD24 markers sorted MCF7 cells into four subsets, i.e., CD44+/CD24-/low, CD44-/CD24+, CD44+/CD24+, and CD44-/CD24-. The SEM data showed that there were many protrusions on the surface of CD44+/CD24-/low cells. CD44+/CD24-/low cells had many microvilli and pseudopodia. The CD44+/CD24-/low cells had a higher migration and invasion abilities than that of the other three subsets of the cells. The in vivo tumor formation assay revealed that CD44+/CD24- cells had the highest tumorigenic capacity compared to the other three subsets.

CONCLUSION

CD44 and CD24 could be useful markers for identification of breast CSCs because CD44+/CD24-/low cells had unique surface ultrastructures and the highest tumorigenicity and invasive abilities.

摘要

背景

鉴定癌症干细胞（CSCs）及其行为将为未来人类癌症的控制提供有见地的信息。本研究在体外和体内研究了 CD44 和 CD24 细胞表面标志物作为乳腺癌 CSC 标志物。

方法

使用 CD44 和 CD24 标记物的流式细胞术对 MCF7 乳腺癌细胞进行分选，用于扫描电子显微镜（SEM）、肿瘤细胞侵袭测定和裸鼠异种移植测定。

结果

使用 CD44 和 CD24 标记物的流式细胞术将 MCF7 细胞分选成四个亚群，即 CD44+/CD24-/low、CD44-/CD24+、CD44+/CD24+和 CD44-/CD24-。SEM 数据显示 CD44+/CD24-/low 细胞表面有许多突起。CD44+/CD24-/low 细胞有许多微绒毛和伪足。CD44+/CD24-/low 细胞的迁移和侵袭能力高于其他三个亚群的细胞。体内肿瘤形成实验表明，与其他三个亚群相比，CD44+/CD24-细胞具有最高的致瘤能力。

结论

CD44 和 CD24 可作为鉴定乳腺癌 CSC 的有用标志物，因为 CD44+/CD24-/low 细胞具有独特的表面超微结构和最高的致瘤性和侵袭能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/764633974347/1475-2867-13-62-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/eef3e8795266/1475-2867-13-62-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/5d844373df66/1475-2867-13-62-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/486d5539b585/1475-2867-13-62-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/764633974347/1475-2867-13-62-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/eef3e8795266/1475-2867-13-62-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/5d844373df66/1475-2867-13-62-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/486d5539b585/1475-2867-13-62-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2330/3717045/764633974347/1475-2867-13-62-4.jpg

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