利用源自空肠弯曲菌 NCTC 11168 的新型 N-乙酰氨基葡萄糖-1-磷酸焦磷酸化酶，通过化学酶法合成非天然糖核苷酸。

A chemoenzymatic route to synthesize unnatural sugar nucleotides using a novel N-acetylglucosamine-1-phosphate pyrophosphorylase from Camphylobacter jejuni NCTC 11168.

机构信息

National Glycoengineering Research Center, Shandong University, Jinan, Shandong 250100, People's Republic of China.

出版信息

Bioorg Med Chem Lett. 2013 Aug 1;23(15):4303-7. doi: 10.1016/j.bmcl.2013.06.003. Epub 2013 Jun 11.

DOI:10.1016/j.bmcl.2013.06.003

Abstract

A novel N-acetylglucosamine-1-phosphate pyrophosphorylase was identified from Campylobacter jejuni NCTC 11168. An unprecedented degree of substrate promiscuity has been revealed by systematic studies on its substrate specificities towards sugar-1-P and NTP. The yields of the synthetic reaction of seven kinds of sugar nucleotides catalyzed by the enzyme were up to 60%. In addition, the yields of the other nine were around 20%. With this enzyme, three novel sugar nucleotide analogs were synthesized on a preparative scale and well characterized.

摘要

从空肠弯曲菌 NCTC 11168 中鉴定出一种新型 N-乙酰氨基葡萄糖-1-磷酸焦磷酸化酶。通过对其对糖-1-P 和 NTP 的底物特异性进行系统研究，揭示了前所未有的底物混杂程度。七种糖核苷酸的合成反应的产率高达 60%。此外，其他九种的产率约为 20%。利用该酶，在制备规模上合成了三种新型糖核苷酸类似物，并对其进行了很好的表征。