Didier A, Decory L, Cazals Y
Laboratoire d'Audiologie Expérimentale, Inserm unité 229, Hôpital Pellegrin, Bordeaux, France.
Hear Res. 1990 Jun;46(1-2):171-6. doi: 10.1016/0378-5955(90)90147-h.
In higher vertebrates, vestibular epithelia contain two types of hair cells, type I and type II cells. Physiological properties of single nerve units have been correlated to the corresponding hair cell type and a better knowledge of the hair cell static and dynamic properties should help in understanding transduction and coding in the vestibular system. For the auditory system, isolated outer cochlear hair cells (OHCs) have provided a useful approach for electrophysiological studies and for observations of motile processes involved in the active control of cochlear micromechanics. The present study tests the hypothesis of motile properties in guinea-pig vestibular hair cells, using stimuli known to elicit motile events in OHCs. After exposure to a medium containing a high concentration in potassium (125 mM or 80 mM KCl), 19 of 26 type I hair cells showed an irreversible tilt of the neck region often accompanied by an obvious swelling of the cell body. In contrast, no shape changes were detected in type II cells. In response to extracellular electrical stimulation, no motility was observed in either type I or type II cells.
在高等脊椎动物中，前庭上皮包含两种类型的毛细胞，即I型和II型细胞。单个神经单元的生理特性已与相应的毛细胞类型相关联，更好地了解毛细胞的静态和动态特性应有助于理解前庭系统中的转导和编码。对于听觉系统，分离的外毛细胞为电生理研究以及观察参与耳蜗微力学主动控制的运动过程提供了一种有用的方法。本研究使用已知能引发外毛细胞运动事件的刺激来检验豚鼠前庭毛细胞运动特性的假设。暴露于含有高浓度钾（125 mM或80 mM KCl）的培养基后，26个I型毛细胞中有19个显示颈部区域出现不可逆的倾斜，且细胞体常伴有明显肿胀。相比之下，未在II型细胞中检测到形状变化。响应细胞外电刺激时，I型和II型细胞均未观察到运动。
