Maness D D, Yakatan G J
J Pharm Sci. 1975 Apr;64(4):651-4. doi: 10.1002/jps.2600640417.
The fluorescence characteristics of 10 substituted 1,4-benzodiazepines in strong acid solution were investigated. The compounds that fluoresce in the Hammett acidity region possess, or can form by enolization, an azomethine linkage in the 1,2- or 4,5-position. All benzodiazepines that fluoresce in strong acid show increases in fluorescence intensity with corresponding blue shifts as acidity increases. Two pKa's in the Hammett acidity region were observed for both the fluorometric and absorptiometric titrations of the benzodiazepines possessing a carbonyl in the 2-position. No evidence of excited state prototropism was observed.
研究了10种取代的1,4 - 苯二氮䓬类化合物在强酸溶液中的荧光特性。在哈米特酸度区域有荧光的化合物在1,2 - 或4,5 - 位具有或可通过烯醇化形成甲亚胺键。在强酸中发荧光的所有苯二氮䓬类化合物随着酸度增加，荧光强度增加且伴有相应的蓝移。对于在2 - 位具有羰基的苯二氮䓬类化合物的荧光滴定和吸收滴定，在哈米特酸度区域观察到两个pKa值。未观察到激发态质子转移的证据。
