Southern Medical University, Nanfang Hospital, Department of Anesthesia, Guangzhou, China, Department of Anesthesia, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Braz J Med Biol Res. 2013 Jun;46(6):507-12. doi: 10.1590/1414-431X20132785. Epub 2013 Jun 25.
Gut-derived endotoxin and pathogenic bacteria have been proposed as important causative factors of morbidity and death during heat stroke. However, it is still unclear what kind of damage is induced by heat stress. In this study, the rat intestinal epithelial cell line (IEC-6) was treated with heat stress or a combination of heat stress and lipopolysaccharide (LPS). In addition, propofol, which plays an important role in anti-inflammation and organ protection, was applied to study its effects on cellular viability and apoptosis. Heat stress, LPS, or heat stress combined with LPS stimulation can all cause intestinal epithelial cell damage, including early apoptosis and subsequent necrosis. However, propofol can alleviate injuries caused by heat stress, LPS, or the combination of heat stress and LPS. Interestingly, propofol can only mitigate LPS-induced intestinal epithelial cell apoptosis, and has no protective role in heat-stress-induced apoptosis. This study developed a model that can mimic the intestinal heat stress environment. It demonstrates the effects on intestinal epithelial cell damage, and indicated that propofol could be used as a therapeutic drug for the treatment of heat-stress-induced intestinal injuries.
肠源性内毒素和致病菌被认为是中暑期间发病率和死亡率升高的重要致病因素。然而，热应激究竟会引起何种损伤仍不清楚。在本研究中，我们采用热应激或热应激联合脂多糖（LPS）处理大鼠肠上皮细胞系（IEC-6），并应用丙泊酚（在抗炎和器官保护方面发挥重要作用）来研究其对细胞活力和细胞凋亡的影响。热应激、LPS 或热应激联合 LPS 刺激均可导致肠上皮细胞损伤，包括早期凋亡和随后的坏死。然而，丙泊酚可以减轻热应激、LPS 或热应激联合 LPS 引起的损伤。有趣的是，丙泊酚仅能减轻 LPS 诱导的肠上皮细胞凋亡，而对热应激诱导的凋亡没有保护作用。本研究建立了一个可模拟肠道热应激环境的模型，证明了其对肠上皮细胞损伤的影响，并表明丙泊酚可用作治疗热应激诱导的肠道损伤的治疗药物。
