USDA Agricultural Research Service, 10300 Baltimore Ave., Bldg. 201 BARC-East, Beltsville, MD 20705, USA.
Meat Sci. 2013 Nov;95(3):603-7. doi: 10.1016/j.meatsci.2013.05.041. Epub 2013 Jun 5.
Scanning electron microscopy (SEM) was utilized to evaluate microstructural changes in intramuscular connective tissue of beef semimembranosus muscle subjected to hydrodynamic pressure processing (HDP). Samples were HDP treated in a plastic container (HDP-PC) or a steel commercial unit (HDP-CU). Control and HDP samples were obtained immediately post-treatment and after 14days of aging for SEM and Warner-Bratzler shear force (WBSF) analysis. Immediately post-treatment, HDP treated samples exhibited lower (P<0.01) WBSF than did controls. After aging, HDP-PC samples had lower (P<0.01) WBSF than that of aged controls. SEM analysis indicated that HDP-PC treatment disrupted the integrity of the collagen fibril network of the endomysium in both the non-aged and aged samples. Aging effects on the intramuscular connective tissue were observed in the HDP-PC and control samples. Both WBSF and connective tissue changes were greater in the HDP-PC than in the HDP-CU treated samples. Data suggest that shockwave alterations to connective tissue contribute to the meat tenderization of HDP.
扫描电子显微镜（SEM）用于评估经流体静压处理（HDP）后的牛肉半膜肌肌内结缔组织的微观结构变化。样品在塑料容器（HDP-PC）或商用钢容器（HDP-CU）中进行 HDP 处理。SEM 和 Warner-Bratzler 剪切力（WBSF）分析采用立即处理后和 14 天老化后的对照和 HDP 样品。处理后立即，HDP 处理的样品的 WBSF 低于对照组（P<0.01）。老化后，HDP-PC 样品的 WBSF 低于老化对照样品（P<0.01）。SEM 分析表明，HDP-PC 处理破坏了非老化和老化样品的肌内膜胶原纤维网络的完整性。在 HDP-PC 和对照样品中观察到了老化对肌内结缔组织的影响。在 HDP-PC 处理的样品中，WBSF 和结缔组织的变化均大于 HDP-CU 处理的样品。数据表明，冲击波对结缔组织的改变有助于 HDP 的肉质嫩化。
