利用外抗原对双极霉属和突脐孢属医学重要分离株进行鉴别
Differentiation of medically important isolates of Bipolaris and Exserohilum with exoantigens.
作者信息
Pasarell L, McGinnis M R, Standard P G
机构信息
Department of Pathology, University of Texas Medical Branch, Galveston 77550.
出版信息
J Clin Microbiol. 1990 Jul;28(7):1655-7. doi: 10.1128/jcm.28.7.1655-1657.1990.
Soluble culture extracts of Bipolaris australiensis, B. hawaiiensis, B. spicifera, Exserohilum longirostratum, E. mcginnisii, E. rostratum, and Helminthosporium solani were used to prepare reference antisera in New Zealand White rabbits. The absorbed reference antisera were tested by a microimmunodiffusion method against concentrated culture filtrates prepared from 115 environmental and clinical isolates of Alternaria spp., Bipolaris spp., Curvularia spp., Dactylaria sp., Drechslera spp., Embellisia spp., Exserohilum spp., Fusarium sp., Helminthosporium sp., Microsporum sp., Scolecobasidium sp., and Scopulariopsis sp. Cross-reactivity did not occur between isolates of the genera tested except for some Bipolaris and Curvularia spp. Antigens shared by species of Bipolaris and Curvularia correlated with their morphologic similarity and phylogenetic closeness. Cross-reactivity was observed among isolates of B. australiensis, B. hawaiiensis, and B. spicifera and among isolates of E. longirostratum, E. mcginnisii, and E. rostratum. The exoantigen test is valuable for differentiating these fungi at the generic level.
用澳大利亚旋孢腔菌、夏威夷旋孢腔菌、稻生旋孢腔菌、长喙突脐孢、麦金尼斯突脐孢、喙突脐孢和茄丝核菌的可溶性培养提取物在新西兰白兔中制备参考抗血清。用微量免疫扩散法对吸收后的参考抗血清进行检测，检测对象为从链格孢属、旋孢腔菌属、弯孢属、指梗霉属、德氏霉属、艾氏霉属、突脐孢属、镰刀菌属、长蠕孢属、小孢子菌属、盾壳霉属和帚霉属的115株环境和临床分离株制备的浓缩培养滤液。除了一些旋孢腔菌属和弯孢属的菌株外，受试属的分离株之间未出现交叉反应。旋孢腔菌属和弯孢属物种共有的抗原与其形态相似性和系统发育亲缘性相关。在澳大利亚旋孢腔菌、夏威夷旋孢腔菌和稻生旋孢腔菌的分离株之间以及长喙突脐孢、麦金尼斯突脐孢和喙突脐孢的分离株之间观察到交叉反应。外抗原试验对于在属水平上区分这些真菌很有价值。
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