Srikanth N S, Seth P K
Developmental Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
J Appl Toxicol. 1990 Jun;10(3):157-60. doi: 10.1002/jat.2550100303.
The effects of endosulfan (3 mg kg-1 body wt., i.p.) and malathion (30 mg kg-1 body wt.) and their coexposure on rat hepatic and brain xenobiotic metabolizing enzymes were investigated. Endosulfan was found to induce aminopyrine-n-demethylase (81%) and aniline hydroxylase (59%) activities significantly in liver and to a lesser extent in brain. Malathion treatment induced malathion carboxylesterase activity in both liver (50%) and brain (22%), significantly depleted liver glutathione (35%) content with stimulation of glutathione-S-transferase (50%) and inhibited the activity of mixed-function oxidases. In the coexposed animals, malathion's inhibitory influence on mixed-function oxidases and endosulfan's inhibitory effect on malathion carboxylesterase were found to dominate, while endosulfan potentiated the activity of glutathione-S-transferase significantly in liver (69%) and brain. A similar trend of alteration in coexposed brain was found, but to a lesser extent. A significant inhibition in brain acetylcholine esterase activity (42%) in the coexposed animals suggests that endosulfan may potentiate the toxicity of malathion by interfering with glutathione and carboxylesterase routes of malathion detoxification.
研究了硫丹（3毫克/千克体重，腹腔注射）、马拉硫磷（30毫克/千克体重）及其共同暴露对大鼠肝脏和脑内异生物质代谢酶的影响。结果发现，硫丹能显著诱导肝脏中氨基比林 - N - 脱甲基酶（81%）和苯胺羟化酶（59%）的活性，在脑中诱导程度较小。马拉硫磷处理可诱导肝脏（50%）和脑中（22%）的马拉硫磷羧酸酯酶活性，显著降低肝脏谷胱甘肽含量（35%），同时刺激谷胱甘肽 - S - 转移酶活性（50%），并抑制混合功能氧化酶的活性。在共同暴露的动物中，发现马拉硫磷对混合功能氧化酶的抑制作用以及硫丹对马拉硫磷羧酸酯酶的抑制作用占主导，而硫丹可显著增强肝脏（69%）和脑中谷胱甘肽 - S - 转移酶的活性。在共同暴露的脑中也发现了类似的变化趋势，但程度较小。共同暴露动物脑中乙酰胆碱酯酶活性显著抑制（42%），这表明硫丹可能通过干扰马拉硫磷解毒的谷胱甘肽和羧酸酯酶途径来增强马拉硫磷的毒性。
