Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Cytotherapy. 2013 Sep;15(9):1073-85. doi: 10.1016/j.jcyt.2013.05.002. Epub 2013 Jun 25.
Cell therapy is considered a promising option for treatment of spinal cord injury (SCI). The purpose of this study is to use combined therapy of bone marrow stromal cells (BMSCs) and BMSC-derived gamma-aminobutyric acid (GABA)ergic inhibitory neurotransmitter cells (BDGCs) for the contusion model of SCI in rats.
BDGCs were prepared from BMSCs by pre-inducing them with β-mercaptoethanol followed by retinoic acid and then inducing them by creatine. They were immunostained with BMSC, proneuronal, neural and GABA markers. The BDGCs were intraspinally transplanted into the contused rats, whereas the BMSCs were delivered intravenously. The animals were sacrificed after 12 weeks.
The Basso, Beattie and Bresnahan test showed improvement in the animals with the combined therapy compared with the untreated animals, the animals treated with GABAergic cells only and the animals that received BMSCs. The immunohistochemistry analysis of the tissue sections prepared from the animals receiving the combined therapy showed that the transplanted cells were engrafted and integrated into the injured spinal cord; in addition, a significant reduction was seen in the cavitation.
The study shows that the combination of GABAergic cells with BMSCs can improve SCI.
细胞疗法被认为是治疗脊髓损伤（SCI）的一种有前途的选择。本研究的目的是使用骨髓基质细胞（BMSCs）和 BMSC 衍生的γ-氨基丁酸（GABA）能抑制性神经递质细胞（BDGCs）联合治疗大鼠 SCI 的挫伤模型。
BDGCs 是通过先用β-巯基乙醇诱导，然后用维甲酸诱导，再从 BMSCs 中制备的。它们用 BMSC、原神经细胞、神经和 GABA 标志物进行免疫染色。BDGCs 被椎管内移植到挫伤大鼠中，而 BMSCs 则静脉内给药。动物在 12 周后被处死。
Basso、Beattie 和 Bresnahan 测试显示，与未治疗动物、仅接受 GABA 能细胞治疗的动物和接受 BMSCs 治疗的动物相比，联合治疗的动物有改善。对接受联合治疗的动物的组织切片进行免疫组织化学分析显示，移植细胞被植入并整合到损伤的脊髓中；此外，空洞明显减少。
该研究表明，GABA 能细胞与 BMSCs 的联合可以改善 SCI。
