Daszkowska-Golec Agata, Chorazy Edyta, Maluszynski Miroslaw, Szarejko Iwona
Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland.
Int J Mol Sci. 2013 Jun 27;14(7):13403-32. doi: 10.3390/ijms140713403.
Abscisic acid plays a pivotal role in the abiotic stress response in plants. Although great progress has been achieved explaining the complexity of the stress and ABA signaling cascade, there are still many questions to answer. Mutants are a valuable tool in the identification of new genes or new alleles of already known genes and in elucidating their role in signaling pathways. We applied a suppressor mutation approach in order to find new components of ABA and abiotic stress signaling in Arabidopsis. Using the abh1 (ABA hypersensitive 1) insertional mutant as a parental line for EMS mutagenesis, we selected several mutants with suppressed hypersensitivity to ABA during seed germination. Here, we present the response to ABA and a wide range of abiotic stresses during the seed germination and young seedling development of two suppressor mutants-soa2 (suppressor of abh1 hypersensitivity to ABA 2) and soa3 (suppressor of abh1 hypersensitivity to ABA 3). Generally, both mutants displayed a suppression of the hypersensitivity of abh1 to ABA, NaCl and mannitol during germination. Both mutants showed a higher level of tolerance than Columbia-0 (Col-0-the parental line of abh1) in high concentrations of glucose. Additionally, soa2 exhibited better root growth than Col-0 in the presence of high ABA concentrations. soa2 and soa3 were drought tolerant and both had about 50% fewer stomata per mm2 than the wild-type but the same number as their parental line-abh1. Taking into account that suppressor mutants had the same genetic background as their parental line-abh1, it was necessary to backcross abh1 with Landsberg erecta four times for the map-based cloning approach. Mapping populations, derived from the cross of abh1 in the Landsberg erecta background with each suppressor mutant, were created. Map based cloning in order to identify the suppressor genes is in progress.
脱落酸在植物的非生物胁迫响应中起关键作用。尽管在解释胁迫和脱落酸信号级联的复杂性方面已取得了很大进展，但仍有许多问题有待解答。突变体是鉴定新基因或已知基因的新等位基因以及阐明它们在信号通路中作用的宝贵工具。我们采用了抑制突变方法来寻找拟南芥中脱落酸和非生物胁迫信号的新组分。以abh1（脱落酸超敏1）插入突变体作为甲基磺酸乙酯诱变的亲本系，我们筛选出了几个在种子萌发期间对脱落酸超敏性受到抑制的突变体。在此，我们展示了两个抑制突变体——soa2（abh1对脱落酸超敏性的抑制子2）和soa3（abh1对脱落酸超敏性的抑制子3）在种子萌发和幼苗发育过程中对脱落酸及多种非生物胁迫的响应。一般来说，在萌发期间，这两个突变体都表现出对abh1对脱落酸、氯化钠和甘露醇超敏性的抑制。在高浓度葡萄糖条件下，这两个突变体都表现出比哥伦比亚-0（Col-0——abh1的亲本系）更高的耐受性。此外，在高脱落酸浓度下，soa2的根生长比Col-0更好。soa2和soa3具有耐旱性，它们每平方毫米的气孔数量比野生型少约50%，但与它们的亲本系abh1相同。考虑到抑制突变体与其亲本系abh1具有相同的遗传背景，为了进行基于图谱的克隆，有必要让abh1与直立型兰茨贝格拟南芥回交四次。构建了源自直立型兰茨贝格拟南芥背景下的abh1与每个抑制突变体杂交的定位群体。基于图谱的克隆以鉴定抑制基因的工作正在进行中。
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