School of Chemistry, University of Birmingham Edgbaston, Birmingham B152TT, UK.
Dalton Trans. 2013 Aug 21;42(31):11220-6. doi: 10.1039/c3dt51090e.
The interaction of salphen-type NiII, CuII and ZnII complexes with native DNA was investigated by exploiting linear dichroism experiments. The NiII complex behaves as a typical intercalator, binding strongly and stiffening and unwinding the DNA. The strength of the DNA interaction is slightly weaker for the copper complex and much weaker for the zinc complex. Plasmid-DNA gel electrophoresis experiments indicated that while CuII and ZnII complexes do not induce the unwinding of supercoiled DNA, the NiII complex has a nuclease activity without the addition of external agents. On the other hand, as shown in the PCR assays, we demonstrate that, at the used concentrations, only the CuII complex is able to inhibit the DNA amplification mediated by Taq DNA polymerase. In this paper we have also reported a detailed characterization of the three compounds including 2D-NMR and ESI-mass experiments and X-ray single crystal structure of the copper and nickel compounds.
利用线二向色性实验研究了水杨醛缩乙二胺型 Ni(II)、Cu(II)和 Zn(II)配合物与天然 DNA 的相互作用。Ni(II)配合物表现为典型的嵌入剂，与 DNA 强烈结合并使 DNA 变僵硬和解旋。铜配合物与 DNA 的相互作用强度稍弱，锌配合物则更弱。质粒-DNA 凝胶电泳实验表明，Cu(II)和 Zn(II)配合物不会诱导超螺旋 DNA 的解旋，而 Ni(II)配合物在没有添加外部试剂的情况下具有核酸酶活性。另一方面，如 PCR 实验所示，我们证明，在所使用的浓度下，只有 Cu(II)配合物能够抑制 Taq DNA 聚合酶介导的 DNA 扩增。在本文中，我们还报道了三种化合物的详细表征，包括 2D-NMR 和 ESI-质谱实验以及铜和镍化合物的 X 射线单晶结构。
