Larson O R, Uglem G L
Department of Biology, University of North Dakota, Grand Forks 58202.
J Parasitol. 1990 Aug;76(4):505-8.
Metacercariae of Clinostomum marginatum obtained from naturally infected Perca flavescens were cultured by 5 methods. In vitro cultivation at 37 C in twice daily changes of Tyrode's, Na-poor Tyrode's, and Eagle's media failed to produce ovigerous adults after 4-5 days. Metacercariae placed on the chorioallantois of week-old chick embryos at 37 C migrated to the albumen. Only 8 of 123 worms recovered were ovigerous after 4-7 days in ovo. Best success occurred with metacercariae injected in groups of 4 or 6 into the abdominal cavities of male A/J mice. Despite liver lesions and strong inflammatory responses in the host, all 174 worms recovered were ovigerous after 4-8 days. None of 41 mice died prematurely from the procedure or the parasites.
从自然感染的黄鲈（Perca flavescens）中获取的边缘斜睾吸虫（Clinostomum marginatum）囊蚴采用5种方法进行培养。在37℃下于泰罗德氏液、低钠泰罗德氏液和伊格尔氏培养基中每日更换两次进行体外培养，4 - 5天后未能产生带卵成虫。将囊蚴置于1周龄鸡胚的尿囊绒膜上，在37℃下会迁移至蛋白中。在卵内培养4 - 7天后，回收的123条虫中只有8条带卵。将囊蚴以每组4条或6条的数量注射到雄性A/J小鼠腹腔内取得了最佳效果。尽管宿主出现肝脏损伤和强烈的炎症反应，但回收的174条虫在4 - 8天后均带卵。41只小鼠中没有一只因该操作或寄生虫而过早死亡。
