Moyano Miguel, Porteros Angel, Dowling John E
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts.
Vis Neurosci. 2013 Jul;30(4):141-5. doi: 10.1017/S0952523813000187. Epub 2013 Jun 28.
Acetylcholine is present in and released from starburst amacrine cells in the inner plexiform layer (IPL), but its role in retinal function except, perhaps, in early development, is unclear. Nicotinic acetylcholine receptors are thought to be present on ganglion, amacrine, and bipolar cell processes in the IPL, and it is known that acetylcholine increases the spontaneous and light-evoked responses of retinal ganglion cells. The effects of acetylcholine on bipolar cells are not known, and here we report the effects of nicotine on the b-wave of the electroretinogram in larval zebrafish. The b-wave originates mainly from ON-bipolar cells, and the larval zebrafish retina is cone-dominated. Only small rod responses can be elicited with dim lights in wild-type larval zebrafish retinas, but rod responses can be recorded over a range of intensities in a mutant ( n o optokinetic response f ) fi sh that has no cone function. We fi nd that nicotine strongly enhances cone-driven b-wave response amplitudes but depresses rod driven b-wave response amplitudes without, however, affecting rod- or cone-driven b-wave light sensitivity.
乙酰胆碱存在于内网状层（IPL）的星爆无长突细胞中并从中释放，但除了可能在早期发育中，其在视网膜功能中的作用尚不清楚。烟碱型乙酰胆碱受体被认为存在于IPL中的神经节细胞、无长突细胞和双极细胞的突起上，并且已知乙酰胆碱会增加视网膜神经节细胞的自发反应和光诱发反应。乙酰胆碱对双极细胞的影响尚不清楚，在此我们报告尼古丁对斑马鱼幼体视网膜电图b波的影响。b波主要起源于ON双极细胞，并且斑马鱼幼体视网膜以视锥细胞为主。在野生型斑马鱼幼体视网膜中，只有昏暗灯光才能诱发微小的视杆细胞反应，但在没有视锥细胞功能的突变体（no optokinetic response f）鱼类中，可以在一系列强度下记录到视杆细胞反应。我们发现尼古丁强烈增强视锥细胞驱动的b波反应幅度，但抑制视杆细胞驱动的b波反应幅度，然而，并不影响视杆细胞或视锥细胞驱动的b波光敏感性。
