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巴西新曲叶黄化病毒的分子和生物学特性。

Molecular and biological characterization of corchorus mottle virus, a new begomovirus from Brazil.

机构信息

Laboratório de Interação Planta-Praga III, Embrapa Recursos Genéticos e Biotecnologia, Pq. Estação Biológica, Brasília, DF, 70770-917, Brazil.

出版信息

Arch Virol. 2013 Dec;158(12):2603-9. doi: 10.1007/s00705-013-1764-x. Epub 2013 Jun 29.

DOI:10.1007/s00705-013-1764-x
PMID:23812656
Abstract

A begomovirus infecting Orinoco jute (Corchorus hirtus) from Brazil was characterized. Molecular analysis revealed a bipartite genomic organization, which is typical of the New World begomoviruses. Sequence analysis and phylogenetic data showed that both genomic components have the closest relationship with abutilon mosaic Brazil virus, with an identity of 87.3 % for DNA-A, indicating that this virus is a member of a new begomovirus species for which the name "Corchorus mottle virus" (CoMoV) is proposed. Sida rhombifolia plants inoculated by biolistics with an infectious clone of CoMoV showed systemic vein chlorosis, mottling and leaf deformation symptoms, while Nicotiana benthamiana and tomato plants had symptomless infection. CoMoV is the first corchorus-infecting begomovirus reported in Brazil.

摘要

一种感染巴西黄麻(Corchorus hirtus)的曲叶病毒被鉴定。分子分析显示其具有二分体基因组结构,这是新世界曲叶病毒的典型特征。序列分析和系统发育数据显示,两个基因组成分与阿布都利通花叶巴西病毒的亲缘关系最近,DNA-A 的同一性为 87.3%,表明该病毒是一种新的曲叶病毒种,建议将其命名为“黄麻斑驳病毒”(CoMoV)。用 CoMoV 的感染性克隆通过生物弹射击导接种 Sida rhombifolia 植株,表现出系统性叶脉褪绿、斑驳和叶片变形症状,而 Nicotiana benthamiana 和番茄植株则无症状感染。CoMoV 是巴西报道的首例感染黄麻的曲叶病毒。

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