经微内窥镜测量的人类伸腕短肌肌节长度。
Sarcomere lengths in human extensor carpi radialis brevis measured by microendoscopy.
机构信息
Department of Mechanical Engineering, Stanford University, Stanford, California, USA.
出版信息
Muscle Nerve. 2013 Aug;48(2):286-92. doi: 10.1002/mus.23760. Epub 2013 Jun 29.
Abstract
INTRODUCTION
Second-harmonic generation microendoscopy is a minimally invasive technique to image sarcomeres and measure their lengths in humans, but motion artifact and low signal have limited the use of this novel technique.
METHODS
We discovered that an excitation wavelength of 960 nm maximized image signal; this enabled an image acquisition rate of 3 frames/s, which decreased motion artifact. We then used microendoscopy to measure sarcomere lengths in the human extensor carpi radialis brevis with the wrist at 45° extension and 45° flexion in 7 subjects. We also measured the variability in sarcomere lengths within single fibers.
RESULTS
Average sarcomere lengths in 45° extension were 2.93±0.29 μm (±SD) and increased to 3.58±0.19 μm in 45° flexion. Within single fibers the standard deviation of sarcomere lengths in series was 0.20 μm.
CONCLUSIONS
Microendoscopy can be used to measure sarcomere lengths at different body postures. Lengths of sarcomeres in series within a fiber vary substantially.
摘要
简介
二次谐波产生微内窥镜是一种微创技术，可在人体中成像肌节并测量其长度，但运动伪影和低信号限制了这项新技术的应用。
方法
我们发现 960nm 的激发波长可最大限度地提高图像信号；这使得图像采集率达到 3 帧/秒，从而减少了运动伪影。然后，我们使用微内窥镜在 7 名受试者中测量了腕部在 45°伸展和 45°弯曲时的人伸腕短肌的肌节长度。我们还测量了单个纤维内肌节长度的变化。
结果
45°伸展时的平均肌节长度为 2.93±0.29μm（±标准差），在 45°弯曲时增加到 3.58±0.19μm。在单个纤维中，肌节长度的系列标准偏差为 0.20μm。
结论
微内窥镜可用于测量不同体位下的肌节长度。纤维内肌节长度的系列变化很大。
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