Institute of Agrochemistry and Food Technology (CSIC), Avda. Agustín Escardino 7, 46980 Paterna (Valencia), Spain.
Int J Food Microbiol. 2013 Aug 1;165(3):339-45. doi: 10.1016/j.ijfoodmicro.2013.06.003. Epub 2013 Jun 14.
Chitosan (CS) films incorporating the antimicrobial compound ethyl-N(α)-dodecanoyl-l-arginate (LAE) were developed for food packaging applications. Cast chitosan films were made with 1, 5 or 10% LAE and 20% glycerol in the film forming solution. Optical properties, release of LAE and antimicrobial activity of developed films was determined. The minimum inhibitory concentration (MIC) and the minimum biocide concentration (MBC) of LAE were determined. CS films with LAE were transparent and uniform, without discontinuities or visible particles and no visual differences could be perceived between CS and CS-LAE films. When in contact with an aqueous food simulant, the agent was fully released following a Fickian behavior in a few hours at 4 and 28°C. Antimicrobial activity of films against mesophiles, psychrophiles, Pseudomonas spp., colifoms, lactic acid bacteria, hydrogen sulfide-producing bacteria, yeast and fungi, was evaluated at two, six and eight days for its application on chicken breast fillets. Films were active against bacteria, yeasts and fungi in liquid and solid media. CS films evidenced antimicrobial activity in the range 0.47-2.96 log reductions, while CS-5%LAE film produced 1.78-5.81 log reduction. Results highlighted that LAE incorporation in a chitosan-based packaging structure may provide a relevant antimicrobial activity that could improve the stability of fresh poultry products.
壳聚糖（CS）薄膜中加入了抗菌化合物 N-（α）-十二酰基-L-精氨酸乙酯（LAE），可用于食品包装应用。在成膜溶液中加入 1%、5%或 10%的 LAE 和 20%的甘油，制备壳聚糖薄膜。测定了所开发薄膜的光学性能、LAE 的释放和抗菌活性。测定了 LAE 的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。含有 LAE 的 CS 薄膜透明且均匀，无中断或可见颗粒，CS 和 CS-LAE 薄膜之间没有明显差异。当与水基食品模拟物接触时，在 4 和 28°C 下，几小时内 agent 以菲克扩散行为完全释放。在 2、6 和 8 天内，评估了薄膜对中温微生物、低温微生物、假单胞菌属、大肠菌群、乳酸菌、产硫化氢细菌、酵母和真菌的抗菌活性，用于鸡胸肉片。薄膜在液体和固体培养基中对细菌、酵母和真菌均具有活性。CS 薄膜在 0.47-2.96 log 减少范围内表现出抗菌活性，而 CS-5%LAE 薄膜则产生 1.78-5.81 log 减少。结果表明，LAE 掺入壳聚糖基包装结构中可能提供相关的抗菌活性，从而提高新鲜禽肉产品的稳定性。
