School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.
Int J Nanomedicine. 2013;8:2227-37. doi: 10.2147/IJN.S42822. Epub 2013 Jun 18.
Post-operatory infections in orthopedic surgeries pose a significant risk. The common approach of using antibiotics, both parenterally or embedded in bone cement (when this is employed during surgery) faces the challenge of the rising population of pathogens exhibiting resistance properties against one or more of these compounds; therefore, novel approaches need to be developed. Silver nanoparticles appear to be an exciting prospect because of their antimicrobial activity and safety at the levels used in medical applications. In this paper, a novel type of silver nanoparticles capped with tiopronin is presented. Two ratios of reagents during synthesis were tested and the effect on the nanoparticles investigated through TEM, TGA, and UV-Vis spectroscopy. Once encapsulated in bone cement, only the nanoparticles with the highest amount of inorganic fraction conferred antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) at concentrations as low as 0.1% w/w. No other characteristics of the bone cement, such as cytotoxicity or mechanical properties, were affected by the presence of the nanoparticles. Our work presents a new type of silver nanoparticles and demonstrates that they can be embedded in bone cement to prevent infections once the synthetic conditions are tailored for such applications.
骨科手术后的感染是一个重大的风险。目前常用的抗生素治疗方法，无论是通过静脉注射还是嵌入骨水泥（在手术中使用），都面临着病原体对一种或多种抗生素产生耐药性的挑战；因此，需要开发新的方法。由于银纳米粒子具有抗菌活性和在医疗应用中使用的安全水平，因此它们似乎是一个令人兴奋的前景。在本文中，提出了一种新型的巯基丙氨酸包覆的银纳米粒子。测试了合成过程中两种试剂的比例，并通过 TEM、TGA 和 UV-Vis 光谱研究了它们对纳米粒子的影响。一旦嵌入骨水泥中，只有无机部分含量最高的纳米粒子在低至 0.1%（重量/重量）的浓度下对耐甲氧西林金黄色葡萄球菌（MRSA）具有抗菌活性。纳米粒子的存在没有影响骨水泥的其他特性，如细胞毒性或机械性能。我们的工作提出了一种新型的银纳米粒子，并证明它们可以嵌入骨水泥中，以防止感染，一旦针对此类应用进行了合成条件的调整。
