Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Int J Phytoremediation. 2013;15(8):729-42. doi: 10.1080/15226514.2012.735286.
The potential of an aquatic fern, Azolla filiculoides, in phytoremediation of a mono azo dye solution, C.I. Acid Blue 92 (AB92), was studied. The effects of operational parameters such as reaction time, initial dye concentration, fern fresh weight, pH, temperature and reusability of the fern on biodegradation efficiency were investigated. The intermediate compounds produced by biodegradation process were analyzed using GC-MS analysis. An artificial neural network (ANN) model was developed to predict the biodegradation efficiency. The findings indicated that ANN provides reasonable predictive performance (R2 = 0.961). The effects of AB92 solutions (10 and 20 mg L(-1)) on growth, chlorophylls and carotenoids content, activity of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase and formation of malondialdehyde were analyzed. AB92 generally showed inhibitory effects on the growth. Moreover, photosynthetic pigments in the fronds significantly decreased in the treatments. An increase was detected for lipid peroxidation and antioxidant enzymes activity, suggesting that AB92 caused reactive oxygen species production in Azolla fronds, which were scavenged by induced activities of antioxidant enzymes.
水蕨在修复单偶氮染料 C.I.酸性蓝 92（AB92）中的应用潜力研究。考察了操作参数，如反应时间、初始染料浓度、蕨类植物鲜重、pH 值、温度和蕨类植物的可重复使用性对生物降解效率的影响。使用 GC-MS 分析对生物降解过程中产生的中间化合物进行了分析。开发了人工神经网络（ANN）模型来预测生物降解效率。研究结果表明，ANN 提供了合理的预测性能（R2 = 0.961）。分析了 AB92 溶液（10 和 20 mg L(-1))对生长、叶绿素和类胡萝卜素含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶等抗氧化酶活性以及丙二醛形成的影响。AB92 通常对生长表现出抑制作用。此外，处理组中叶片中的光合色素显著减少。脂质过氧化和抗氧化酶活性的增加表明，AB92 在水蕨叶片中产生了活性氧，抗氧化酶的诱导活性清除了这些活性氧。
