Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Z Naturforsch C J Biosci. 2013 Mar-Apr;68(3-4):108-12.
Cytotoxic activity of gaillardin, a sesquiterpene lactone isolated from Inula oculus-christi L. (Asteraceae), was assessed in the human breast adenocarcinoma cell line MCF-7, human hepatocellular carcinoma cell line HepG-2, human non-small cell lung carcinoma cell line A-549, and human colon adenocarcinoma cell line HT-29, resulting in IC50 values of 6.37, 6.20, 4.76, and 1.81 microg/mL, respectively, in the microculture tetrazolium-formazan MTT assay. In vitro apoptosis-inducing properties of gaillardin were also evaluated in MCF-7 cells with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. The results suggest gaillardin as a candidate for further studies in cancer therapy
从蓝刺头（菊科）中分离出的倍半萜内酯 Gaillardin 对人乳腺癌细胞 MCF-7、人肝癌细胞 HepG-2、人非小细胞肺癌细胞 A-549 和人结肠腺癌细胞 HT-29 的细胞毒活性进行了评估，在微量细胞培养噻唑蓝比色（MTT）测定中，IC50 值分别为 6.37、6.20、4.76 和 1.81μg/mL。Gaillardin 在 MCF-7 细胞中的体外诱导细胞凋亡特性也通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记（TUNEL）试验进行了评估。结果表明，Gaillardin 是癌症治疗进一步研究的候选药物。
