Department of Biology, Vassar College , Poughkeepsie, New York.
J Neurotrauma. 2013 Nov 15;30(22):1919-24. doi: 10.1089/neu.2013.2951. Epub 2013 Sep 27.
Astrogliosis occurs following injury to the zebra finch brain. To date, only estrogen synthase (aromatase) has been identified in injury-induced astrocytes. The expression of other steroidogenic enzymes or their receptors remains unknown in the avian brain. However, in mammals, an upregulation of androgen receptors has been identified in glial cells. The aim of this study was to determine if the androgen receptor is upregulated following injury in adult zebra finches. Finches were given a single penetrating injury and brain tissue was collected 24 or 72 h later. Expression of androgen receptor was examined using immunohistochemistry and quantified using quantitative polymerase chain reaction (qPCR) analysis. Androgen receptors were localized to astrocytes versus neurons, further solidifying the role for astrocytes in neural recovery.
脑损伤会引起星型胶质细胞增生。迄今为止，只有雌激素合成酶（芳香酶）在损伤诱导的星形胶质细胞中被鉴定出来。在禽类大脑中，其他类固醇生成酶或其受体的表达仍然未知。然而，在哺乳动物中，已经发现神经胶质细胞中的雄激素受体上调。本研究的目的是确定成年斑马雀在受伤后雄激素受体是否上调。给雀鸟注射单一的穿透性损伤，然后在 24 或 72 小时后收集脑组织。使用免疫组织化学法检查雄激素受体的表达，并使用定量聚合酶链反应 (qPCR) 分析进行定量。雄激素受体定位于星形胶质细胞而非神经元，进一步证实了星形胶质细胞在神经恢复中的作用。
