Fischl Wolfgang, Bartenschlager Ralf
Haplogen GmbH, Vienna, Austria.
Methods Mol Biol. 2013;1030:205-19. doi: 10.1007/978-1-62703-484-5_17.
Large-scale antiviral drug discovery and systematic identification of host factors promoting or restricting virus replication require robust, scalable, and cost-effective assay systems that allow for high throughput and automation. Here we describe the construction and application of full-length infectious dengue virus reporter genomes for such screening applications.
大规模抗病毒药物研发以及对促进或限制病毒复制的宿主因子进行系统鉴定，需要强大、可扩展且具有成本效益的检测系统，以实现高通量和自动化。在此，我们描述了用于此类筛选应用的全长感染性登革病毒报告基因基因组的构建和应用。
