Arnaud F G, Pegg D E
MRC Medical Cryobiology Group, University Department of Surgery, Cambridge, UK.
Thromb Res. 1990 Mar 15;57(6):919-24. doi: 10.1016/0049-3848(90)90158-9.
We have previously described a laboratory technique for the cryopreservation of human platelets with 1.4 molar glycerol. For the present study we adapted that method for use in clinical blood banks, and we describe the results of in vitro assays of platelets preserved in PVC packs, with storage at -75 degrees C for 1 month. The results suggested that substitution of the PVC pack for standard, 4 ml polypropylene freezing vials had little effect, but substitution of processing (addition and subsequent removal of glycerol) in PVC packs for processing in polystyrene containers resulted in a marked reduction in recovery. The final results, where the entire process was carried out in PVC packs was not satisfactory; only approximately 50% of the platelets were recovered and they exhibited only approximately 30% hypotonic stress response and approximately 3% aggregation response to ADP. We conclude that -75 degrees C may be too high a storage temperature, or that the specific blood packs that we used may be unsuitable for this process, or both.
我们之前描述了一种使用1.4摩尔甘油冷冻保存人血小板的实验室技术。在本研究中，我们将该方法应用于临床血库，并描述了保存在聚氯乙烯（PVC）袋中、于-75摄氏度储存1个月的血小板的体外检测结果。结果表明，用PVC袋替代标准的4毫升聚丙烯冷冻瓶影响不大，但在PVC袋中进行处理（添加和随后去除甘油）替代在聚苯乙烯容器中处理会导致回收率显著降低。整个过程都在PVC袋中进行的最终结果并不理想；仅约50%的血小板被回收，并且它们对低渗应激的反应仅约为30%，对二磷酸腺苷（ADP）的聚集反应约为3%。我们得出结论，-75摄氏度可能是过高的储存温度，或者我们使用的特定血袋可能不适合此过程，或者两者皆是。
